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Effects of delaying the addition of BMP2 on E16 cortical cell differentiation. Cells were grown at low density with FGF2 alone for the first 4 div, and then BMP2 was added at 10 or 100 ng/ml. The number of cells expressing neuronal (β tubulin III), oligodendroglial (O4), astrocytic (GFAP), and neuroepithelial (nestin) markers was quantitated at 8 div. *p < 0.05; **p < 0.01.
