Table 1.
Clonal analysis of E16 cortical cells grown in FGF2 alone or with BMP2
| FGF2 | FGF2 + BMP2 | |
|---|---|---|
| Fate of individual cells: | n = 663 | n = 816 |
| Nonviable | 276 (41.6) | 378 (46.3) |
| Single cells | 252 (38) | 252 (30.9) |
| Neurons | 224 (88.9) | 240 (95.2) |
| Clones containing 2–4 cells | 39 (5.9) | 36 (4.4) |
| with neurons | 26 (66.7) | 32 (88.9) |
| Clones containing >4 cells | 96 (14.5) | 150 (18.4) |
| with neurons | 9 (10.7) | 109 (72.7) |
| with neurons and OLs | 41 (42.7) | 0 (0) |
| with OLs | 37 (38.5) | 0 (0) |
| without neurons or OLs | 9 (10.7) | 41 (27.3) |
| Cellular composition within clones: | ||
| 2–4 Cell clones with neurons, % neurons | 100 | 96.9 |
| >4 Cell clones with neurons, % neurons | 3.6 ± 1.2 | 20.5 ± 3.4** |
| >4 Cell clones with OLs, % OLs | 16.4 ± 2.8 | 1-a |
| Viability within clones | ||
| Average # of viable cells per >4 cell clone | 102.9 ± 11.9 | 70.3 ± 10.8 |
| Average # of nonviable cells per >4 cell clone | 14.4 ± 2.0 | 18.2 ± 2.0 |
Clonal analysis of E16 cortical cells grown in FGF2 alone or FGF2 plus 1 ng/ml BMP2. Fate of individual cells, Each absolute value is followed by a percentage in parentheses, which reflects the percentage of all cells in that particular category. Cellular composition within clones, Viability within clones, Each value represents the mean ± SEM. Neurons and oligodendroglia were identified by β tubulin III and O4 expression, respectively. Cells expressing the astrocytic marker GFAP were not included because they were never seen as single cells or in small clones and were present in <1% of clones of more than four cells.
No clones containing oligodendroglia were seen with BMP2 treatment.
*p < 0.05; **p < 0.01.