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. 1999 Nov 1;19(21):9618–9634. doi: 10.1523/JNEUROSCI.19-21-09618.1999

Fig. 1.

Fig. 1.

Purification of GAPRFVamide from the CNS ofAplysia using immunoassay. A, First separation step of reversed-phase HPLC. The column (Capcell Pak C18; 10 × 250 mm) was developed by 0–60% acetonitrile and 0.1% TFA. A shaded bar indicates the immunoreactive fraction from which GAPRFVamide was purified. B, Second step of cation-exchange HPLC. The column (TSKgel SP-5PW; 7.5 × 75 mm) was eluted with a gradient of 0–0.6 m NaCl and 10 mm sodium phosphate buffer, pH 6.7) A shaded bar indicates the immunoreactive fraction that was subjected to the next HPLC step. C, Final step of reversed-phase HPLC. The column (TSKgel ODS-80TM; 4.6 × 150 mm) was eluted by 15–25% acetonitrile and 0.1% TFA. Arrow indicates a peak of the purified peptide.