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Potentiation of IEG expression in the ACC after the amputation. Photomicrographs showing expression of c-Fos and NGFI-A (A) and phosphorylation of CREB (B) in the coronal ACC sections from sham animals (Ctrl) and animals at different times after the amputation of the unilateral hindpaw third digit. Scale bars, 500 μm. The numbers of c-Fos-, NGFI-A-, and pCREB-immunoreactive cells increased bilaterally after the amputation (see Table 1 for summary).
