Fig. 6.

MEQ fluorescent cells within area CA1 pyramidal cell layer exclude PI 10 min after H₂O₂. An MEQ-loaded slice was constantly superfused with PI.A, MEQ fluorescent neurons in area CA1 before H₂O₂. B, Neurons inA imaged with the rhodamine filter for PI.C, MEQ fluorescent neurons 10 min after H₂O₂. D, Neurons inC imaged with the rhodamine filter for PI.E, As a positive control, the slice was exposed to methanol for 2 min to allow entry of PI into the same cells shown inA–D. All images were at a 1.7× electronic zoom to maintain registry between multiple laser lines. Scale bar, 20 μm.