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. 1999 Nov 1;19(21):9530–9537. doi: 10.1523/JNEUROSCI.19-21-09530.1999

Fig. 8.

Fig. 8.

RT-PCR–Southern blot analysis of gene expression in eyeblink conditioned rabbit. Top, Expression of Rbt KKIAMRE in the cerebellar deep nuclei and cerebellar cortex. A 399 bp RT-PCR product was hybridized with a 677 bp cDNA fragment in the 3′ noncoding region. Lanes U1 and U2represent RNA from rabbits trained with unpaired stimuli andlanes P1 and P2 RNA from well conditioned rabbits. For semiquantitative analysis, serial dilutions of the control cDNA plasmid was used for PCR: 2 × 106 copies (lane 1), 2 × 105 copies (lane 2), 2 × 104 copies (lane 3), 2 × 103 copies (lane 4), 2 × 102 copies (lane 5), and 2 × 101 copies (lane 6). PCR was performed for 30 cycles.Bottom, Expression of a control gene NF-L using the same RNA samples as in A. A 699 bp fragment was amplified from 27 PCR cycles and hybridized with an 855 bp cDNA probe in the coding region. Copy number of plasmids used for positive control PCR (lanes 1–6) was the same as mentioned above.