Skip to main content
. 1999 Nov 1;19(21):9160–9169. doi: 10.1523/JNEUROSCI.19-21-09160.1999

Fig. 7.

Fig. 7.

Cell death induced by LY 294002 shared apoptotic features with that observed after MEX deprivation.A, MTNs were maintained in culture in the presence of MEX for 48 hr. Then, cells were grown for 15 hr in different culture conditions: basal medium without any trophic support (NE); supplemented with MEX; or supplemented with 10 ng/ml GDNF, NTN, or PSP in the presence or absence of 50 μm LY 294002. After this time, MTN nuclei were stained with Hoechst. Representative photomicrographs of cultures treated with basal medium (NE), MEX, GDNF, or GDNF plus LY 294002. B, Percentage of apoptotic cells in each condition. Quantification of apoptotic nuclei was performed as described in Materials and Methods. The percentages in control cultures were 8.5 ± 2.7 for NE and 5.9 ± 1.3 for MEX.C, Assay of caspase activity performed in cultures treated for 8 hr with 10 ng/ml GDNF, NTN, or PSP in the presence (open bars) or absence (filled bars) of 50 μm LY 294002, MEX, or basal medium (NE). At the end of the treatment, cells were lysed, and protein extracts were assayed for its caspase activity as described in Materials and Methods. Results are expressed as pmol of AFC · min-1 · μg of protein.Asterisk indicates significant differences (p < 0.01) on caspase activity between GDNF-, NTN-, or PSP-treated cultures and NE- or GDNF, NTN, or PSP plus LY 294002-treated cultures.