Table 2.
TBARS levels in neuronal cultures exposed to copper or iron
| TBARS (OD Units × 10−3/well) | ||
|---|---|---|
| WT | APP−/− | |
| No treatment | 4 ± 3 | 6 ± 4 |
| CuCl2(10 μm) | 41 ± 7* | 27 ± 8 |
| CuCl2 (100 μm) | 50 ± 3* | 41 ± 5 |
| FeCl2 (100 μm) | 40 ± 8 | 37 ± 8 |
| FeCl2 (1000 μm) | 75 ± 4 | 77 ± 9 |
WT and APP−/− cortical neuronal cultures were exposed to metals at indicated concentrations for 16 hr at day 6in vitro. TBA solution was added to neurons and media and incubated at 95°C for 20 min. The values represent optical density (OD) units (× 10−3) per well (from 24 well plates) after correction for control media alone. Copper, but not iron, induced significantly greater levels of TBARS in WT compared to APP−/− cultures.
*p < 0.05.