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Micro-CALI of the extracellular domain of all isoforms of NCAM using MG-labeled 5e does not affect growth cone behavior. A growth cone was incubated with MG-labeled 5e and observed by video time-lapse microscopy (A). A region of the growth cone is chosen for micro-CALI (white outline) and laser irradiated for 5 min (B, C). D,The growth cone is observed for an additional 5 min. Micro-CALI did not cause filopodial or lamellipodial retraction. Scale bar, 10 μm.
