Fig. 7.

Micro-CALI of NCAM-180 and L1 simultaneously causes both neurite retraction and growth cone collapse. DRG neurons were loaded with MG-labeled 4d and incubated with MG-labeled 8D9. After observation for 10 min (a), the growth cone was irradiated for 5 min, starting at t = 0 (b, black outline in b) and ended at t = +5 min (c,white outline). Shortly thereafter, the filopodia and lamellipodia retract, and the growth cone continues to collapse, but the neurite does not decrease in length (d). Over the next 10 min the neurite retracts (e) and begins to recover after an additional 10 min (f). Scale bar, 10 μm.