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Inhibition of binding of 125I-NGF to TrkA-BALB/C 3T3 cells. Hybridoma supernatants were preincubated with TrkA-BALB/C 3T3 cells before the addition of 125I-NGF. The histogram reports the inhibition of specific NGF binding to TrkA-BALB/C 3T3 cells by different antibodies. Specific binding was evaluated by subtracting from the total binding that obtained in the presence of an excess of unlabeled NGF. The values reported are the mean ± SEM of triplicates.
