Fig. 2.

Adult human white matter harbors oligodendrocyte progenitors. Immunocytochemistry of white matter dissociates for a panel of cell type-selective antigens revealed a diverse representation of phenotypes before sorting. A–C, A typical bipolar cell, double-labeled for A2B5 (red) and BrdU (yellow), fixed after 4 DIV. D–F, A cluster of postmitotic O4⁺ cells (D, E) and an overtly less mature BrdU-incorporating O4⁺/BrdU⁺ cell (F), all fixed after 7 DIV. G–I, Representative examples of the diverse phenotypes present in the adult white matter. These included cells expressing CNP (G), GFAP (H), and TuJ1 (I) immunoreactivities, which respectively identify oligodendrocytes, astrocytes, and neurons; each cell type was found in the proportion noted in Results. Scale bar, 40 μm.