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Culture, isolation, and enrichment of oligodendrocyte progenitors. Adult human subcortical white matter, derived from surgical samples of frontal and temporal lobe, was dissected and enzymatically dissociated using papain and DNase and then cultured and transfected with either P/hCNP2:hGFP or control plasmids (P/CMV:hGFP and P/hCNP2:lacZ).
