Fig. 4.

A, Synaptic currents recorded in the presence of 10 μm bicuculline at membrane potentials of −60, −40, −20, 0, and +10 mV using low-chloride internal solution. B, Normalized current–voltage relationship for IPSCs recorded in 10 μm bicuculline using low-chloride (closed circles) (n = 4) and high-chloride internal solutions (open squares) (n = 6). The reversal potential in low-chloride internal was −44 mV, whereas in high-internal chloride it was 0 mV.C, The GABA uptake blocker NO-711 slows the decay of IPSCs. Each panel shows a train of five IPSCs at stimulated at 20 Hz. Application of NO-711 slows the decay of the synaptic current; after washout of NO-711, the decay returns back to the control response.