Fig. 2.

Sox10 transactivates the β4 and α3 gene promoters in a cell type-specific manner. A, B, The β4/luciferase construct pX1B4FH (A) and the α3/luciferase construct pX1A3HS (B) were transiently transfected into neuronal (Neuro2A and SN17) and non-neuronal (Sol8 muscle, NIH3T3, and Rat2 fibroblast) cell lines either alone (−) or with pCMV5 (pCMV) or pCMVSox10 (Sox10). Luciferase values were normalized to β-galactosidase expression as driven by the SV40 promoter. Error bars represent SDs. C, Qualitative Western blot analysis of transfected cells is shown. Cell extracts were prepared from untransfected cells or cells transfected with pCMV5 or pCMVSox10. For a given cell line, equivalent amounts of protein for each condition were analyzed by SDS-PAGE. After SDS-PAGE, Western blot analysis was done using anti-Sox10 antibody to demonstrate Sox10 expression in pCMVSox10-transfected cells.