Photograph of an adult short-tailed opossum,Monodelphis domestica (a), and a P4 infant (b). To document the surgical procedures, a CCD camera (Optronics) was used to produce digital images of the P4 infants while they were attached to the mother's nipple during our procedure (c). A midline incision was made, and the skin and skull were retracted in preparation for the cortical neuroepithelial reduction. After the lesion was made with the excision tool (d), the skin was repositioned with forceps and held together with glue. Scale bar, 1 mm.