Fig. 2.
Dual in situ hybridization to show coexpression of α1b ADR mRNA in c-fos mRNA-containing cells of the PVN 30 min after IL-1β (5 μg/kg, i.p.). A, C, c-fos mRNA in the PVN, labeled with a nonradioactive (digoxigenin) probe, viewed under bright-field illumination. B, D, α1b ADR mRNA in the PVN, labeled with a radioactive (35S) probe, viewed under dark-field illumination. The box in B represents the approximate area over which cells were counted on each side of the PVN.Arrows in C and D indicate examples of double-labeled cells. Scale bars: A, B, 400 μm; C, D, 50 μm.