Table 2.
Levels of α1b ADR mRNA in the hippocampus, dorsal raphe, and lateral amygdala after bilateral ADX or SHAM surgery
| Group | α1b ADR mRNA: % signal w.r.t. SHAM | n | ||
|---|---|---|---|---|
| Hippocampus | Dorsal raphe | Lateral amygdala | ||
| Naive | 103 ± 8 | 99 ± 5 | 96 ± 10 | 4 |
| UNOP | 102 ± 5 | 98 ± 21 | 91 ± 7 | 3 |
| SHAM, 1 d | 100 ± 7 | 100 ± 8 | 100 ± 2 | 3 |
| SHAM, 3 d | 100 ± 13 | 100 ± 9 | 100 ± 8 | 3 |
| SHAM, 7 d | 100 ± 5 | 100 ± 7 | 100 ± 3 | 3 |
| SHAM, 14 d | 100 ± 10 | 100 ± 5 | 100 ± 5 | 3 |
| ADX, 1 d | 96 ± 9 | 109 ± 8 | 99 ± 7 | 4 |
| ADX, 3 d | 108 ± 12 | 96 ± 15 | 97 ± 2 | 4 |
| ADX, 7 d | 113 ± 7 | 104 ± 10 | 94 ± 3 | 4 |
| ADX, 14 d | 96 ± 2 | 117 ± 10 | 94 ± 3 | 4 |
Animals were killed 1, 3, 7, or 14 d after surgery. In addition, naive animals that had not experienced any experimental manipulation and UNOP animals, which did not undergo surgery, but were given saline–dextrose solution to drink, were included. Levels of mRNA were set at 100% for each SHAM group, and data are expressed as mean percentage of signal (relative optical density above background; see Materials and Methods for more detail) ± SEM with respect to (w.r.t.) appropriate SHAM group. Naive and UNOP groups are expressed w.r.t. the mean of all SHAM animals. There were no significant differences in expression levels between groups as determined by one-way ANOVA.