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. 1999 Oct 1;19(19):8476–8486. doi: 10.1523/JNEUROSCI.19-19-08476.1999

Fig. 5.

Fig. 5.

Effects of NRTN on the survival of E9 ciliary and trigeminal ganglion neurons. A, Dissociated E9 ciliary ganglion neurons grown in the presence of 50 ng/ml NRTN.B, Dissociated E9 ciliary ganglion neurons grown in the absence of trophic factors. C, Dissociated E9 trigeminal ganglion neurons grown for 2 d in the presence of 50 ng/ml NRTN and subsequently stained for Ret. All cells with neuronal morphology are positive with Ret staining (arrows), whereas flat non-neuronal cells are devoid of staining. A neuron probed with the primary antibody that was preincubated with a blocking peptide shows no staining (inset). Scale bar, 50 μm. D, Survival of E9 ciliary ganglion neurons in the presence of 50 ng/ml NRTN or CNTF in comparison with control (no factor). E, Survival of E9 trigeminal ganglion neurons in the presence of 50 ng/ml NRTN or BDNF in comparison with control. Results are expressed as the percentage of the neurons that survived after 48 hr in culture (± SE). Note that the majority of E9 ciliary ganglion neurons is supported by NRTN (D), whereas only a subpopulation of E9 trigeminal ganglion neurons is NRTN-dependent (E).