Fig. 2.

Dps is proteolytically cleaved. Immunoblot analysis of second instar larval extracts from wild type (wt) and mutants indicates that Dps is proteolytically cleaved to give rise to a 25 kDa N-terminal fragment (a) and a 35 kDa C-terminal fragment (b) that are absent from the deletion mutants W6 and W11. Full-length protein can only be detected in third instar larval extracts of transgenic flies that overexpress Dpsfrom a heatshock promoter (hs-Dps) or use a daughterless-GAL4 driver (da-Dps) (c). In the absence of GAL4, only the N-terminal fragment is detected with the N-terminal-specific Dps antibody.