Fig. 3.
Dps mutants fail to undergo proper neuronal differentiation within the optic lobe anlagen and the eye imaginal disk. a, Wild-type CNS and disks derived from late third larval instar and double-immunostained for CUT (green) and ELAV (red). Note the precise laminar array organization of the optic lobe anlagen (OL) and the regular array of neurons within the developing eye imaginal disk (longarrow). At this stage, CUT expression is also abundant in developing antennal disk (shortarrow). b, DAPI staining of disks demonstrating the overall morphology of wild-type eye (arrow) and antennae (arrowhead) disks. c, Wild-type wing imaginal disks immunostained for WINGLESS. Note the normal distribution of wingless in the dorsal region of the wing disk in regions that will give rise to the adult notum and hinge (h) and the ventral staining in regions of the wing blade (wb) that will give rise to the wing margin. d, CNS and imaginal disks fromDps46/DpsW11 late third larval instar. Note the general disorganization of the optic lobe anlagen (OL) and the absence of ELAV expression in the eye imaginal disk (longarrow). In contrast, expression of CUT in the antennal disk appears normal (shortarrow). e, DAPI staining of disks fromDps46/DpsW11mutants. Although the antennae disk (arrowhead) appears normal, the eye disk (arrow) is small and disorganized. f, Wing imaginal disks fromDps46/DpsW11. WINGLESS expression appears normal in the dorsal compartment in regions that will give rise to the notum and hinge (h) but is absent from ventral regions that give rise to the wing blade and margin (wb).