Fig. 6.

Notch staining is increased on the plasma membrane in the second larval instar CNS of Dps mutants. a, In wild-type second larval instar CNS, Notch expression is observed throughout the cytoplasm of neuroblasts and at high levels at the plasma membrane in regions of contact between the neuroblast and its progeny (arrow). b, c, In the CNS of Dps mutants, Notch expression is increased on the entire surface of the plasma membrane (thickarrows) and at the regions of contact between cells (thinarrow). d, Processing of Notch to give rise to a functional heterodimeric receptor is unaffected inDps mutants. Protein extracts were obtained from isolated CNS and imaginal disks from wild-type and Dps homozygous mutant third larval instars. No significant differences were observed in the levels or processing of Notch in Dps mutants compared with that of wild type.