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. 1999 Oct 1;19(19):8498–8508. doi: 10.1523/JNEUROSCI.19-19-08498.1999

Fig. 5.

Fig. 5.

Inhibition by cycloheximide of the neuregulin-mediated increase in the ε-mRNA. C2C12 myotubes were treated with or without 10 μg/ml cycloheximide (CH) for 30 min before neuregulin stimulation for the indicated times. Total RNA was isolated for Northern blot analysis using the respective probe for the ε-subunit or c-fos. The loading was uniform as evidenced by an equal amount of GAPDH mRNA.A, Representative Northern blot radiograms of the ε-mRNA. B, Histograms showing mean ± SD of three different samples. The mRNA levels in the absence of neuregulin were considered to be 100% for the indicated time treatment. **p < 0.01 in comparison with the neuregulin-mediated increase in the absence of cycloheximide.C, Representative Northern blot radiograms of thec-fos mRNA.