Fig. 3.

SRF protein expression is upregulated by SE. Representative Western blots using pilocarpine- and saline-treated rat nuclear-enriched fractions obtained 24 hr (A;n = 10) and 8 weeks (B;n = 4) after injection. SRF immunoreactive bands were visualized using an anti-SRF antibody, a peroxidase-conjugated secondary antibody, and enhanced chemiluminescence. The mature form of SRF is shown at 68 kDa, and the immature, newly synthesized form is at 60 kDa. Quantitation of the mature SRF band at both time points by densitometry (C) revealed a significant increase in SRF protein. Error bars represent SEM; *p < 0.05; **p < 0.01; Student’s ttest.