Fig. 4.

Long-term alterations in specific binding of SRF to its SRE DNA element in nuclear-enriched fractions from specific brain regions of epileptic animals. Hippocampal (n= 6), cortical (n = 6), and cerebellar (n = 5) nuclear-enriched fractions were prepared from pilocarpine-treated epileptic (P) and saline-treated control (C) rats 8 weeks after treatment. EMSAs were performed as in Figure 2. A representative EMSA using these nuclear-enriched fractions is shown (A). Quantitation of these gels and determination of percent change in SRE binding over paired control nuclear-enriched fractions for three brain regions (B) revealed a significant increase in SRF binding in the hippocampus and cortex. Error bars represent SEM; *p < 0.05; **p < 0.01; Student’s ttest.