Fig. 6.

Upregulation of specific SRF DNA binding at 8 weeks after SE was prevented by NMDA channel inhibition during SE. MK-801, an NMDA channel antagonist, was administered before pilocarpine or saline injection. Nuclear-enriched fractions prepared 8 weeks after treatment from four groups of rats [Pilo/Saline(n = 6) (Fig. 2), Pilo/MK-801(n = 4), Saline/MK-801(n = 4), and Saline/Saline(n = 6)]. Electrophoretic mobility-shift assays were conducted and quantitated as described in Figure 2. Data are represented as a percent change in SRE binding from control animals (Saline/MK-801; n = 4), and controls (Saline/Saline). Error bars represent SEM; **p < 0.01; Student’s ttest.