Fig. 2.
Association of α-synuclein with PKC isoforms in rat brain tissue. Left panels show immunoblots of α-synuclein immunoprecipitates with isoform-specific PKC antibodies. Right panels show immunoblots of each PKC isoform in the membrane and cytoplasmic fractions. Before each immunoprecipitation, half of the brain homogenates were treated with 1 μm PMA plus 1 mm Ca2+ at 30°C for 30 min. The homogenates were then fractionated into membrane and cytoplasmic components taken up in immunoprecipitation buffer, and proteins were isolated as shown. Cytoplasmic, C; membrane, M; omit, Ø, which is an immunoprecipitation done using protein A but no 1° antibody.