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. 1999 Jul 15;19(14):5990–6005. doi: 10.1523/JNEUROSCI.19-14-05990.1999

Fig. 9.

Fig. 9.

GFAP was used to label astrocytes within the graft areas (red) to assess the extent of colocalization with BrdU-labeled transplanted cells (green).A, Cells within the RMS at 6 weeks after transplantation were often closely associated with GFAP profiles, although no double-labeled cells were observed (arrows).B, Within the granule cell layer of the olfactory bulb, BrdU-positive cells were interspersed with, but not colocalized with, GFAP (arrows). C, In the dentate gyrus, cells within the transplant core were occasionally closely associated with GFAP-positive cytoplasmic staining, possibly indicating a double-labeled cell. D, Staining within the striatum revealed a dense network of GFAP-positive processes (red) intermingled with the BrdU-positive cells (green). Many clear examples of double-labeled cells were observed (arrowheads), although examples of BrdU single-labeled cells were also frequently observed (arrows). Insets show BrdU/GFAP double-labeled cells at higher magnification. Scale bar (shown inA): A, C, 50 μm; B, 25 μm; D, 150 μm.