Table 1.
VEGF (ng/ml) | SCG | DRG | ||||
---|---|---|---|---|---|---|
Treated | Control | n | Treated | Control | n | |
1 | +++ | ++ | 6 | nd | nd | |
10 | +++ | ++ | 3 | + | + | 6 |
50 | ++++ | ++ | 9 | + | + | 6 |
100 | ++++ | ++ | 5 | + | + | 6 |
Axonal outgrowth, i.e. number of axons, determined by ocular evaluation after 48 hr in culture. The experiment was repeated three times except for the concentrations of 10 and 100 ng/ml on SCG, which were repeated twice. For ranking see Materials and Methods subheading Axonal outgrowth. nd, Not determined; n, number of animals.