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. 2019 Sep 9;8:e48629. doi: 10.7554/eLife.48629

Figure 3. Kinesin detaches from MTs when encountering antibody obstacles.

(A) Kinesin and dynein were labeled with organic dyes and their motility was tested in the presence and absence of anti-tubulin antibody on MTs. (B) Kymographs of TMR-kinesin and LD555-DDB walking on MTs in the absence and presence of 20 µg/mL antibody obstacle. (C) Quantification of how antibody obstacles affect motor motility. All data are normalized to the no antibody condition (mean ± SD, two independent experiments). From left to right, n = 185, 232, 199, 197 motors for kinesin and 104, 224, 262, 308 motors for DDB.

Figure 3.

Figure 3—figure supplement 1. Larger obstacles block access to more protofilaments on an MT.

Figure 3—figure supplement 1.

The schematics represent the cross-sectional view of a motor bypassing an obstacle by taking sideways steps on an MT. Increasing the size of the obstacle or the cargo attached to the motor is expected to reduce the number of protofilaments that support motility (white). The protofilaments that are blocked by the obstacle/cargo are shown in red.