Video 2. Kinesin motors walk processively in the absence of QD obstacles, but motility was completely impaired in the presence of 25 nM QDs.

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DOI: 10.7554/eLife.48629.005

Kinesin motors were labeled with QD655 at their C-termini. Single-molecule motility of kinesin in the presence of no obstacles (top) or 25 nM QD585 obstacles (bottom) on surface-immobilized MTs. The fluorescence signal of QD585 obstacles was collected in a separate channel, and not displayed. The sample was excited with a 1.7 kW cm⁻²488 nm laser beam under the epifluorescence mode. Images were acquired at 10 Hz.