Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Strain, strain background (Vibrio cholerae)	V. cholerae O1 El Tor, strain A1552 (Primary strain)	PMID: 9473029 PMID: 30574591 (genome sequence)		See Supplementary file 1 for strains and plasmids used in this study
Strain, strain background (Vibrio cholerae)	V. cholerae, strain Sa5Y (Secondary strain)	PMID: 17449702 PMID: 30574591 (genome sequence)		See Supplementary file 1 for strains and plasmids used in this study
Other	TCBS agar, selective medium	Sigma-Aldrich	86348–500G	Additional, standard growth media are described under growth conditions
Peptide, recombinant protein	Pwo SuperYield DNA Polymerase	Roche / Sigma-Aldrich	4340850001
Peptide, recombinant protein	GoTaq G2 DNA Polymerase	Promega	M7848
Commercial assay or kit	Genomic-tip 100/G (DNA purification)	Qiagen	10243
Commercial assay or kit	Genomic DNA Buffer Set	Qiagen	19060