Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus, C57BL/6J) | Wildtype C57BL/6 (WT) | Jackson Laboratories | Stock # 000664 | |
| Strain, strain background (M. musculus, C57BL/6J) | Complement C3 deficient (C3-/-) | Jackson Laboratories | Stock # 029661 | |
| Strain, strain background (M. musculus, C57BL/6J) | T cell receptor alpha deficient (TCRα-/-) | Jackson Laboratories | Stock # 002116 | |
| Strain, strain background (M. musculus, C57BL/6J) | Transgenic OVA-specific CD4 T cells (OT-II) | Jackson Laboratories | Stock # 004194 | |
| Strain, strain background (M. musculus, C57BL/6J) | Transgenic Csf1r-eGFP (MAFIA) | Jackson Laboratories | Stock # 005070 | |
| Strain, strain background (M. musculus, C57BL/6J) | C3.SW-H2b | Jackson Laboratories | Stock # 000438 | |
| Strain, strain background (Herpes simplex virus type 1, McKrae) | HSV-1 | Macdonald et al., 2012; Watson et al., 2012 | N/A | Originally from Brian Gebhardt |
| Cell line (Cercopithecus aethiops) | Vero | American Type Culture Collection (ATCC) | Cat. # CCL-81 | see Materials and methods |
| Chemical compound, drug | Cobra Venom Factor, Naja naja kaouthia | Millipore-Sigma | Cat. # 233552 | |
| Commercial assay or kit | Anti-mouse CD90.2 IMag particles | BD | Cat. # 551518 | |
| Commercial assay or kit | Anti-mouse CD4 microbeads | Miltenyi Biotec | Cat. # 130-049-201 | |
| Commercial assay or kit | Anti-mouse CD8 microbeads | Miltenyi Biotec | Cat. # 130-116-478 | |
| Commercial assay or kit | Anti-mouse CD90.2 microbeads | Miltenyi Biotec | Cat. # 130-049-101 | |
| Commercial assay or kit | Anti-mouse CD45 microbeads | Miltenyi Biotec | Cat. # 130-052-301 | |
| Commercial assay or kit | Anti-mouse EpCAM microbeads | Miltenyi Biotec | Cat. # 130-105-958 | |
| Antibody | Rabbit polyclonal IgG (anti-mouse beta-III tubulin) | Abcam | Cat. # ab18207 | (1:500) |
| Antibody | AlexaFluor647 donkey polyclonal IgG (anti-rabbit IgG) |
Jackson Immunoresearch | Cat. # 711-605-152 | (1:1000) |
| Antibody | Anti-mouse CD3e FITC (Clone: 145–2 C11) |
eBioscience (ThermoFisher) | Cat. # 11-0031-82 | (1:500) |
| Other | Roche Liberase TL | Sigma Aldrich | Cat. # 5401020001 | see Materials and methods |
| Commercial assay or kit | C3 ELISA | Abcam | Cat. # ab157711 | |
| Commercial assay or kit | Milliplex MAP Luminex Array | EMD Millipore | Custom Design | |
| Recombinant DNA reagent | iScript cDNA synthesis kit | Biorad | Cat. # 1708891 | |
| Recombinant DNA reagent | Sso Advanced SYBR Green qPCR Supermix | Biorad | Cat. # 1725270 | |
| Other | Trizol | ThermoFisher | Cat. # 15596026 | see Materials and methods |
| Software, algorithm | Prism 6 | GraphPad | N/A | |
| Software, algorithm | Imaris x64 (8.2.1) | Bitplane | N/A |