Table 2. Agreement between experimental and back-calculated intermolecular distances for different hexamer structures.

RMS values are shown comparing the measured versus the predicted distances from each structural model for distances measured from S33, L54 and S61. Data from position S20 were not used as they arise from non-specific interactions with MTSL. See also Figure 5—figure supplement 3.

PRE term	Hexamer 1 RMS (Å)	Hexamer 2(i) RMS (Å)	Hexamer 2(ii) RMS (Å)	Hexamer 2(iii) RMS (Å)
S33C(ΔN6)-ΔN6	2.34	2.68	2.58	2.53
L54C(ΔN6)-ΔN6	1.25	2.33	2.26	1.87
S61C(ΔN6)-ΔN6	2.22	2.7	2.68	3.11