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. 2019 Sep 25;8:e47534. doi: 10.7554/eLife.47534

Table 1. (p)ppGpp binding and inhibition of HPRT homologs.

Organism Relative activityd Kd (pppGpp) (μM) IC50 (pppGpp)
(μM)
25 μM ppGpp 25 μM pppGpp
Actinobacteria
Streptomyces coelicolor −0.01 ± 0.01 0.12 ± 0.01 1.77 ± 0.31 3.8 ± 0.2
Collinsella aerofaciens 0.31 ± 0.04 0.42 ± 0.05 1.46 ± 0.56
Cellulomonas gilvus NAb NAb 0.6 ± 0.11
Mycobacterium tuberculosis NAa NAa NAc
Bacteroidetes
Bacteroides intestinalis 0.49 ± 0.06 0.05 ± 0.01 0.11 ± 0.03
Bacteroides caccae 0.34 ± 0.03 0.01 ± 0.02 0.11 ± 0.02
Bacteroides ovatus 0.43 ± 0.04 −0.03 ± 0.02 0.15 ± 0.04
Bacteroides uniformis 0.4 ± 0.01 0.01 ± 0.01 0.1 ± 0.04
Bacteroides thetaiotaomicron 0.41 ± 0 0 ± 0.01 0.11 ± 0.04
Bacteroides finegoldii 0.46 ± 0.03 −0.01 ± 0.01 0.15 ± 0.06
Firmicutes
Eubacterium ventriosum −0.03 ± 0.03 −0.07 ± 0.03 0.66 ± 0.1
Ruminococcus lactaris −0.01 ± 0 0 ± 0 0.11 ± 0.03
Ruminococcus torques NAb NAb 0.22 ± 0.07
Clostridium leptum −0.01 ± 0.01 0 ± 0 3.29 ± 0.94
Eubacterium eligens −0.03 ± 0.03 0.02 ± 0.01 0.79 ± 0.18
Blautia hansenii 0.07 ± 0 0.14 ± 0.01 0.83 ± 0.14
Eubacterium hallii 0.17 ± 0.05 −0.06 ± 0.05 0.07 ± 0.02
Clostridium spiroforme 0.07 ± 0.01 0.17 ± 0 9.54 ± 1.17
Listeria monocytogenes 0.13 ± 0 0.32 ± 0.02 0.32 ± 0.06
Bacillus subtilis 0.08 ± 0.01 0.41 ± 0.01 0.75 ± 0.07 10.1 ± 1.3
Staphylococcus aureus 0.31 ± 0.01 0.48 ± 0.01 2.83 ± 0.38 10.6 ± 0.3
Streptococcus mutans NAb NAb 5.12 ± 1.45
Bacillus anthracis 1 0.25 ± 0.01 0.5 ± 0.01 1.54 ± 0.18 29.5 ± 1.2
Enterococcus faecalis 0.31 ± 0.01 0.49 ± 0.02 1.12 ± 0.11
Holdemania filiformis 0.66 ± 0.01 0.43 ± 0 0.17 ± 0.11
Bacillus anthracis 2 0.81 ± 0.04 0.7 ± 0.05 6.18 ± 1.77
Deinococcus-Thermus
Thermus thermophilus 0.52 ± 0.07 −0.01 ± 0.01 0.04 ± 0.01
Proteobacteria
Caulobacter crescentus 0.33 ± 0.05 0.47 ± 0.07 0.24 ± 0.09
Escherichia coli 0.33 ± 0.01 0.48 ± 0.02 3.38 ± 1.47 26.2 ± 2.0
Klebsiella pneumoniae 0.47 ± 0.03 0.49 ± 0.01 3.38 ± 0.89
Sinorhizobium meliloti NAb NAb 3.08 ± 0.92
Yersinia enterocolitica 0.7 ± 0.01 0.7 ± 0.03 4.44 ± 1.25
Photobacterium phosphoreum 0.97 ± 0.01 0.96 ± 0.03 NAc >100
Pseudomonas syringae 0.6 ± 0.01 0.6 ± 0.01 5.85 ± 1.76
Pseudomonas aeruginosa 0.84 ± 0.01 0.83 ± 0.02 5.28 ± 1.52
Legionella pneumophila 0.78 ± 0.01 0.69 ± 0.03 1.72 ± 0.38 >100
Coxiella burnetii 0.79 ± 0.14 0.72 ± 0.16 NAc >100
Neisseria meningitidis 0.9 ± 0.02 0.8 ± 0.03 1.64 ± 0.55
Eukarya
Saccharomyces cerevisiae 0.64 ± 0.02 0.96 ± 0.01 8.08 ± 8.58
Caenorhabditis elegans NAb NAb 12.77 ± 7.68 29.6 ± 2.8
Homo sapiens NA NA NA 66.6 ± 5.4

± standard error of the mean.

NA = no data obtained.

aProtein not purified.

bProtein did not have activity.

cInteraction with pppGpp too weak to estimate Kd.

dRelative to activity without ppGpp or pppGpp.