Table 4. HPRT kinetic parameters.
| HPRT | Km (guanine)
(μM) |
Km (PRPP)
(μM) |
kcat
(s−1) |
|---|---|---|---|
| B. anthracis 1 | 5.2 ± 1.8 | 111.6 ± 16.8 | 22 |
| B. subtilis | -- | 165.6 ± 11.9 | 25.4 |
| C. burnetii | 17.6 ± 2.0 | 44 ± 3.3 | 12.9 |
| P. phosphoreum | -- | 81 ± 12.5a | 30.4a |
| N. meningitidis | 68 ± 7.0 | 103 ± 12 | 9.6 |
| E. colib | -- | 192 ± 7.0a | 59a |
| L. pneumophilac | 10.5 ± 1.1 | 60 ± 9 | -- |
| M. tuberculosisd | 10 ± 1 | 650 ± 70 | 0.193 |
PRPP = phosphoribosyl pyrophosphate.
aobtained with hypoxanthine as the purine base.
± standard error.