Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Rattus norvegicus domestica) | wildtype wistar rat pups | CEMT, University, Freiburg | ||
| Genetic reagent | AAV9.CAG.GCaMP6s.WPRE.SV40 | Penn Vector Core, University of Pennsylvania | V3296TI-R | titer 1e11 |
| Antibody | anti-MAP2 (chicken polyclonal) | Abcam, Cambridge, UK | ab92434 RRID:AB_2138147 | 1:500 |
| Antibody | anti-NeuN (rabbit polyclonal) | Abcam, Cambridge, UK | ab128886 RRID:AB_2744676 |
1:500 |
| Antibody | anti-Neurofilament (mouse monoclonal) | Abcam, Cambridge, UK | ab24571 RRID:AB_448148 |
1:10 |
| Antibody | anti-Synapsin (mouse monoclonal) | Synaptic Systems GmbH, Germany | 106001 RRID:AB_887805 |
1:200 |
| Antibody | anti-chicken-Cy2 (goat polyclonal) | Abcam, Cambridge, UK | ab6960 RRID:AB_955003 |
1:200 |
| Antibody | anti-rabbit-Cy3 (goat polyclonal) | Abcam, Cambridge, UK | ab6939 RRID:AB_955021 |
1:200 |
| Antibody | anti-mouse-Cy5 (goat polyclonal) | Abcam, Cambridge, UK | ab6563 RRID:AB_955068 |
1:200 |
| Chemical compound, drug | 4,6-diamidino-2-phenyindole, diclactate (DAPI) | Sigma-Aldrich, Germany | D9562 | 1:5000 |
| Chemical compound, drug | Gödecke6976 | Tocris Bioscience, Bristol, UK | 2253 | 1 µM |
| Chemical compound, drug | Phorbol-12-Myristate-13-Acetate (PMA) | Sigma-Aldrich, Munich, Germany | P1585 | 1 µM |
| Chemical compound, drug | Picrotoxin | Tocris Bioscience, Bristol, UK | 1128 | 10 µM |
| Chemical compound, drug | DMSO | Sigma-Aldrich, Munich, Germany | D8418 | 0.1% |
| Chemical compound, drug | DNase (type IV) | Sigma-Aldrich, Munich, Germany | D5025 | 50 g/ml |
| Chemical compound, drug | minimal essential medium | Invitrogen, Karlsruhe, Germany | 21090055 | |
| Chemical compound, drug | horse serum (heat-inactivated) | Invitrogen, Karlsruhe, Germany | 26050088 | 20% |
| Chemical compound, drug | phosphate buffered saline (PBS) | Invitrogen, Karlsruhe, Germany | 21600010 | |
| Chemical compound, drug | glucose | Sigma-Aldrich, Munich, Germany | G7528 | 20 mM |
| Chemical compound, drug | L-glutamine | Invitrogen, Karlsruhe, Germany | 25030024 | 0.5 mM |
| Chemical compound, drug | gentamycin | Invitrogen, Karlsruhe, Germany | 15750060 | 20 µg/ml |
| Chemical compound, drug | potassiumD-gluconate | Sigma-Aldrich, Munich, Germany | G4500 | 125 mM |
| Chemical compound, drug | EGTA | Carl Roth, Karlsruhe, Germany | 3054 | 5 mM |
| Chemical compound, drug | KCl | Sigma-Aldrich, Munich, Germany | P4504 | 20 mM |
| Chemical compound, drug | Na2-ATP | Carl Roth, Karlsruhe, Germany | K054 | 2 mM |
| Chemical compound, drug | Hepes | Carl Roth, Karlsruhe, Germany | 9105 | 10 mM |
| Chemical compound, drug | CaCl2 | Sigma-Aldrich, Munich, Germany | C3881 | 0.5 mM |
| Chemical compound, drug | KOH | Sigma-Aldrich, Munich, Germany | P4504 | |
| Chemical compound, drug | MgCl2 | Sigma-Aldrich, Munich, Germany | MO250 | 2 mM |
| Software | MC Rack software | Multi Channel Systems, Germany | versions 3.3–4.5 RRID:SCR_014955 |
|
| Software | Spike2 software | Cambridge Electronics Design Ltd., Cambridge, UK. | RRID:SCR_000903 | |
| Software | Zen | Carl Zeiss, Jena, Germany | RRID:SCR_013672 | |
| Software | MEA-Tools | Egert et al., 2002 (PMID 12084562) | version 2.8 | |
| Software | FIND toolbox | Meier et al., 2008 (PMID 18692360) | ||
| Software | ImageJ | Schneider et al., 2012 (PMID 22930834) | RRID:SCR_003070 | |
| Software | Matlab | Mathworks, Natick, MA, USA | versions 2014a – 2017a |