Table 1. Morphometric analysis of network development under different PKC conditions.
Results are presented as mean ± standard error of mean (SEM). Significance was determined against PKCN, or between specified developmental time windows, using independent Student’s t-test. N specifies the number of analyzed images taken from two networks per PKC condition and age.
Table 1—source data 1. Source data and Matlab script.
elife-47996-table1-data1.zip (5.9KB, zip)
DOI: 10.7554/eLife.47996.015
Table 1—source data 2. Source data and Matlab script.
elife-47996-table1-data2.zip (43.4KB, zip)
DOI: 10.7554/eLife.47996.016
| DIV | PKC- | PKCN | PKC+ | unit | |
|---|---|---|---|---|---|
| clustering index | |||||
| 8 | 0.92 ± 0.01 (1.8*10−10) | 0.84 ± 0.01 | 0.82 ± 0.02 (6.1*10−1) | CI | |
| 15 | 0.9 ± 0.01 (1.5*10−6) | 0.73 ± 0.01 | 0.69 ± 0.02 (1.1*10−1) | CI | |
| 22 | 0.88 ± 0.01 (4.4*10−4) | 0.75 ± 0.03 | 0.67 ± 0.02 (3.3*10−2) | CI | |
| 29 | 0.89 ± 0.01 (8.0*10−9) | 0.79 ± 0.01 | 0.67 ± 0.02 (2.1*10−5) | CI | |
| 8 vs. 22 | −4.49 (3.5*10−4) | −9.65 (3.1*10−2) | −18.6 (1.2*10−5) | % change | |
| 22 vs. 29 | 1.04 (4.9*10−1) | 4.5 (2.7*10−1) | 0.01 (1.0) | % change | |
| Dendrite size | |||||
| 8 | 476 ± 9 (1.3*10−3) | 421 ± 12 | 408 ± 8 (3.4*10−1) | µm | |
| 15 | 797 ± 22 (1.2*10−2) | 676 ± 37 | 610 ± 30 (2.1*10−1) | µm | |
| 22 | 1413 ± 64 (7.9*10−5) | 1021 ± 41 | 816 ± 24 (3.6*10−4) | µm | |
| 29 | 1380 ± 74 (1.9*10−4) | 962 ± 65 | 760 ± 37 (1.3*10−2) | µm | |
| 8 vs. 22 | 196.59 (4.0*10−20) | 142.46 (9.1*10−12) | 100.08 (1.4*10−15) | % change | |
| 22 vs. 29 | −2.38 (7.4*10−1) | −5.81 (5.0*10−1) | −6.86 (2.5*10−1) | % change | |
| Synapse density | |||||
| 8 | 281 ± 11 (2.2*10−1) | 255 ± 18 | 254 ± 14 (9.4*10−1) | # | |
| 15 | 1142 ± 44 (2.3*10−4) | 754 ± 39 | 510 ± 9 (2.4*10−5) | # | |
| 22 | 2188 ± 100 (2.1*10−5) | 1427 ± 99 | 885 ± 27 (3.4*10−5) | # | |
| 29 | 2019 ± 110 (4.5*10−8) | 1114 ± 56 | 669 ± 21 (5.7*10−8) | # | |
| 8 vs. 22 | 678.77 (4.7*10−24) | 458.69 (2.1*10−10) | 248.68 (1.2*10−17) | % change | |
| 22 vs. 29 | −7.75 (2.7*10−1) | −21.93 (6.7*10−3) | −24.35 (1.2*10−6) | % change | |
| Dendritic occupancy | |||||
| 8 | 0.59 ± 0.02 (6.4*10−1) | 0.6 ± 0.04 | 0.62 ± 0.03 (7.5*10−1) | #/µm | |
| 15 | 1.44 ± 0.05 (1.7*10−2) | 1.13 ± 0.11 | 0.85 ± 0.04 (1.7*10−2) | #/µm | |
| 22 | 1.55 ± 0.03 (9.6*10−2) | 1.4 ± 0.09 | 1.09 ± 0.04 (5.7*10−3) | #/µm | |
| 29 | 1.47 ± 0.04 (3.0*10−5) | 1.19 ± 0.04 | 0.9 ± 0.04 (2.3*10−5) | #/µm | |
| 8 vs. 22 | 163.83 (7.2*10−28) | 132.26 (9.0*10−8) | 76.65 (3.7*10−10) | % change | |
| 22 vs. 29 | −5.06 (1.5*10−1) | −15.41 (2.3*10−2) | −17.45 (3.8*10−3) | % change | |
| Neuron density | |||||
| 8 | 255 ± 6 (9.6*10−7) | 185 ± 11 | 168 ± 7 (2.0*10−1) | #/mm2 | |
| 15 | 214 ± 9 (5.9*10−4) | 131 ± 17 | 158 ± 12 (2.0*10−1) | #/mm2 | |
| 22 | 107 ± 8 (1.9*10−1) | 123 ± 6 | 85 ± 6 (5.7*10−4) | #/mm2 | |
| 29 | 87 ± 5 (3.0*10−1) | 96 ± 7 | 77 ± 4 (2.6*10−2) | #/mm2 | |
| 8 vs. 22 | −58.03 (7.3*10−17) | −33.66 (1.1*10−4) | −49.42 (1.0*10−8) | % change | |
| 22 vs. 29 | −18.93 (4.5*10−2) | −21.71 (1.3*10−2) | −9.79 (2.6*10−1) | % change | |
| Maximum connectivity | |||||
| 8 | 0.01 ± 0.001 (3.5*10−2) | 0.013 ± 0.001 | 0.014 ± 0.001 (6.3*10−1) | fraction | |
| 15 | 0.048 ± 0.003 (4.6*10−1) | 0.053 ± 0.006 | 0.029 ± 0.002 (9.2*10−4) | fraction | |
| 22 | 0.209 ± 0.031 (8.2*10−3) | 0.104 ± 0.007 | 0.098 ± 0.009 (5.9*10−1) | fraction | |
| 29 | 0.229 ± 0.026 (9.2*10−4) | 0.116 ± 0.014 | 0.081 ± 0.006 (3.8*10−2) | fraction | |
| 8 vs. 22 | 1987.43 (5.9*10−10) | 701.18 (2.6*10−11) | 604.32 (1.4*10−10) | % change | |
| 22 vs. 29 | 9.31 (6.3*10−1) | 11.48 (5.2*10−1) | −17.23 (1.3*10−1) | % change | |
| N | |||||
| 8 | 24 | 11 | 15 | ||
| 15 | 9 | 4 | 7 | ||
| 22 | 15 | 11 | 11 | ||
| 29 | 17 | 16 | 15 |