Table 2. Electrophysiological characterization of network activity during development.
Data were pooled within defined developmental time windows. Significance was determined against PKCN using independent Student’s t-test. N specifies the number of recorded networks per PKC condition and age.
| DIV | PKC− | PKCN | PKC+ | |
|---|---|---|---|---|
| AFR | ||||
| 3–5 | 0.03 ± 0.01 (1.0*10−1) | 0.05 ± 0.01 | 0.09 (1.4*10−1) | |
| 6–9 | 0.08 ± 0.01 (2.6*10−3) | 0.18 ± 0.03 | 0.1 ± 0.02 (4.0*10−2) | |
| 10–14 | 0.36 ± 0.04 (1.8*10−2) | 0.49 ± 0.03 | 0.42 ± 0.04 (2.0*10−1) | |
| 15–20 | 0.53 ± 0.06 (3.6*10−1) | 0.61 ± 0.06 | 0.76 ± 0.19 (3.6*10−1) | |
| 21–27 | 0.69 ± 0.08 (2.5*10-1) | 0.8 ± 0.06 | 1.21 ± 0.12 (7.3*10−4) | |
| 28–35 | 0.6 ± 0.07 (2.9*10−3) | 0.96 ± 0.09 | 1.41 ± 0.15 (6.9*10−3) | |
| 36–44 | 0.42 ± 0.08 (2.4*10−7) | 1.18 ± 0.1 | 2.04 ± 0.83 (4.3*10−2) | |
| 45+ | 0.24 ± 0.02 (9.9*10−8) | 1.06 ± 0.14 | 1.2 ± 0.63 (8.3*10−1) | |
| SBE rate (SBE/min) | ||||
| 3–5 | 0.17 ± 0.04 (2.9*10−1) | 0.11 ± 0.03 | 0.54 (1.5*10−2) | |
| 6–9 | 0.18 ± 0.03 (5.4*10−6) | 1.02 ± 0.15 | 1.46 ± 0.19 (7.4*10−2) | |
| 10–14 | 1.21 ± 0.13 (2.0*10−8) | 4.26 ± 0.44 | 11.69 ± 1.27 (3.4*10−10) | |
| 15–20 | 2.83 ± 0.35 (1.4*10−8) | 6.36 ± 0.43 | 14.31 ± 2.03 (4.7*10−7) | |
| 21–27 | 4.58 ± 0.44 (2.4*10−10) | 10.3 ± 0.62 | 26.82 ± 3 (5.5*10−12) | |
| 28–35 | 4.98 ± 0.81 (2.2*10−13) | 16.97 ± 1.07 | 41.1 ± 5.07 (7.9*10-9) | |
| 36–44 | 4.08 ± 0.75 (9.0*10−12) | 17.25 ± 1.28 | 26.21 ± 7.05 (8.7*10−2) | |
| 45+ | 3.74 ± 0.56 (2.2*10−13) | 18.85 ± 1.62 | 45.76 ± 23.86 (2.0*10−3) | |
| SBE strength (APs per burst) | ||||
| 3–5 | 6.2 ± 3 (1.0*100) | 6.2 ± 3.8 | 3.5 (7.6*10−1) | |
| 6–9 | 21.6 ± 2.1 (9.4*10−7) | 9.5 ± 1 | 4.6 ± 0.6 (1.2*10−3) | |
| 10–14 | 21.2 ± 2.1 (5.7*10−9) | 9 ± 0.8 | 2.7 ± 0.5 (1.5*10−7) | |
| 15–20 | 15.2 ± 1.9 (2.1*10−3) | 8 ± 1.3 | 2.7 ± 0.3 (1.2*10−2) | |
| 21–27 | 10.1 ± 1 (6.1*10−8) | 4.9 ± 0.4 | 4.4 ± 0.9 (5.4*10−1) | |
| 28–35 | 8.9 ± 0.9 (2.9*10−6) | 4 ± 0.5 | 2.3 ± 0.3 (1.7*10−2) | |
| 36–44 | 6.2 ± 0.6 (2.2*10−1) | 5.1 ± 0.6 | 5.1 ± 1.6 (9.8*10−1) | |
| 45+ | 5.6 ± 0.8 (4.2*10−2) | 3.8 ± 0.5 | 1.1 ± 0.4 (2.2*10−1) | |
| PFR (Hz) | ||||
| 3–5 | 12.3 ± 3.8 (9.0*10−1) | 13.2 ± 7.3 | 11.5 (9.2*10−1) | |
| 6–9 | 50.8 ± 4.8 (6.4*10−5) | 28.3 ± 2.7 | 17.4 ± 1.7 (4.9*10−3) | |
| 10–14 | 76.6 ± 5.4 (6.7*10−14) | 32.1 ± 2.3 | 10.3 ± 1.4 (2.1*10−9) | |
| 15–20 | 59.1 ± 6.5 (5.3*10−5) | 29.8 ± 3.4 | 10.9 ± 1.3 (6.4*10−4) | |
| 21–27 | 43.3 ± 4.1 (7.4*10−10) | 18.9 ± 1.5 | 13.7 ± 2 (4.4*10−2) | |
| 28–35 | 42.3 ± 4.4 (2.4*10−8) | 15.5 ± 2 | 8.1 ± 1.2 (1.8*10−2) | |
| 36–44 | 30.5 ± 3.1 (2.7*10−2) | 21.4 ± 2.6 | 6.1 ± 0.4 (1.3*10−1) | |
| 45+ | 27.5 ± 3.8 (1.5*10−2) | 16.4 ± 2.3 | 5.6 ± 1.7 (2.8*10−1) | |
| Network synchrony | ||||
| 3–5 | 0.1 ± 0.03 (2.6*10−1) | 0.04 ± 0.02 | 0.08 (3.4*10−1) | |
| 6–9 | 0.39 ± 0.02 (3.3*10−3) | 0.29 ± 0.02 | 0.15 ± 0.02 (3.1*10−4) | |
| 10–14 | 0.52 ± 0.03 (2.5*10−10) | 0.31 ± 0.02 | 0.12 ± 0.02 (1.4*10−10) | |
| 15–20 | 0.53 ± 0.04 (4.6*10−5) | 0.35 ± 0.02 | 0.16 ± 0.03 (1.7*10−5) | |
| 21–27 | 0.51 ± 0.03 (5.8*10−13) | 0.26 ± 0.02 | 0.2 ± 0.02 (4.8*10−2) | |
| 28–35 | 0.57 ± 0.04 (2.0*10−10) | 0.24 ± 0.03 | 0.15 ± 0.03 (7.6*10−2) | |
| 36–44 | 0.53 ± 0.04 (1.3*10−5) | 0.3 ± 0.03 | 0.11 ± 0.03 (1.3*10−1) | |
| 45+ | 0.45 ± 0.05 (2.5*10−3) | 0.26 ± 0.03 | 0.14 ± 0.11 (3.8*10−1) | |
| N | ||||
| 3–5 | 7 | 3 | 1 | |
| 6–9 | 33 | 40 | 24 | |
| 10–14 | 70 | 92 | 47 | |
| 15–20 | 53 | 65 | 27 | |
| 21–27 | 77 | 121 | 56 | |
| 28–35 | 47 | 62 | 29 | |
| 36–44 | 38 | 57 | 4 | |
| 45+ | 38 | 36 | 2 | |