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. Author manuscript; available in PMC: 2019 Oct 22.
Published in final edited form as: Curr Biol. 2018 Oct 11;28(20):3212–3219.e4. doi: 10.1016/j.cub.2018.08.028

Figure 3. The fitness benefit of aggregation is lost in mutagenized populations.

Figure 3

A. Whole-well fluorescence microscopy of MEFs inoculated with aggregated VSV-GFP and monodisperse VSV-mCherry particles (1), and with monodisperse VSV-GFP and aggregated VSV-mCherry particles (2). VSV-GFP and VSV-mCherry were mutagenized prior to competition assays using 5-FU. Images were taken at 34 hpi and correspond to one of the three replicate assays. The fraction of total fluorescent area occupied by GFP-positive cells is shown in the right graph for the three replicates. Error bars: SEM. B. Growth curve of VSV in MEFs by real-time whole-well fluorescence microscopy. The percentage of fluorescent cells in the well is shown. Grey: non-mutagenized, monodisperse inoculum. Orange: mutagenized, monodisperse inoculum. Red: mutagenized, aggregated inoculum. Each data point is the average of three replicates. Error bars: SEM.