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. Author manuscript; available in PMC: 2019 Oct 8.
Published in final edited form as: Immunometabolism. 2019 Sep 25;1:e190014. doi: 10.20900/immunometab20190014

Figure 2. Activated T cells support increased metabolism and function of CD25high tumour interacting NK cells.

Figure 2

Cultured NK cells (6 days in IL15 10 ng/mL) were purified and then co-cultured with B16 melanoma cells and purified T cells at NK:T:B16 ratio of 1:1:2. The T cells were either naïve or αCD3/αCD28-activated T cells. The NK cells were analysed by flow cytometry for cell size and CD71 (a,b), NKp46 expression (c) IFNγ production and Gnzb expression (a,d,e) in CD25low and CD25high NK cells as indicated. Data is representative (a–e) or mean ± SEM (b–e) of 4 independent experiments. Data was analyzed using a paired students t-test. (* p < 0.05, *** p < 0.001, ns non-significant).