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. Author manuscript; available in PMC: 2019 Oct 8.
Published in final edited form as: Immunometabolism. 2019 Sep 25;1:e190014. doi: 10.20900/immunometab20190014

Figure 6. cMyc is required for NK1.1 plus IL2-induced NK cell metabolism.

Figure 6

(a–e) Cultured (6 days in IL15 10 ng/mL) from cMycfl/fl Tamox-Cre and cMycwt/wt Tamox-Cre were treated with tamoxifen (0.6 μM) and were purified and stimulated with a plate bound α-NK1.1 antibody (10 μg/mL) plus IL2 (20 ng/mL) for 18 h. Cells were analysed by flow cytometry for cMyc and CD71 expression (a,c) or by quantitative rtPCR for cMyc mRNA expression (b). Alternatively, rates of OXPHOS (d) or glycolysis (e) were measured using the seahorse extracellular flux analyser. Data is representative (a,c,d,e) or mean ± SEM (a,b,d,e) of 3 independent experiments. Data was analyzed using a students t-test (a) or a one sample t-test against a theoretical value of 1 (b,d,e) (* p < 0.05, ** p < 0.01).