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. Author manuscript; available in PMC: 2020 Oct 7.
Published in final edited form as: Dev Cell. 2019 Aug 22;51(1):113–128.e9. doi: 10.1016/j.devcel.2019.07.022

Figure 7. YAP loss does not induce apoptosis or acinar re-differentiation in poorly-differentiated, p53 deficient pancreatic tumor cells.

Figure 7.

(A-B) Fold difference in median CellROX fluorescence (A) and percentage of apoptotic cells as determined by TUNEL assay (B) at 5 days post infection with Ad-GFP or Ad-CRE in KYY and KPYY pancreatic tumor cells. n = 3.

(C) Changes in the ratios of KPYY cells pretreated with GFP+ or Tm+ and co-cultured over indicated time and analyzed periodically by flow cytometry. n = 3.

(D) Western blot analysis of Sox2, Myc and Yap proteins in Yap− KPYY cells at indicated days post infection of Ad-GFP or Ad-CRE. Vinc was used as the loading control. Shown is representative of at least three independent experiments.

(E) Quantification and representative flow cytometry plots of Annexin-V/CYTOX-Blue staining of parental or crispr-Sox2-knockout (crSox2) KPYYF cells at 5 days post infection post infection with Ad-GFP or Ad-CRE. n = 3.

(F) Representative IHC images of Yap, Ki67, Casp3, Myc, Sox2, and Amy of orthotopic KPYY pancreatic tumors that were either untreated (−TAM) or treated with TAM for one week (ST) or > 2 months (LT). Scale bar = 100 μm.

**P < 0.005. ***P < 0.0005. ns: not significant. Error bars indicate s.d.