Table IV:
Top 20 upstream regulators not associated with Type I IFN, as based on impact of T-cell depletion on –log (adjusted p-values)
| CD4 top upstream regulators | CD8 top upstream regulators | |||
|---|---|---|---|---|
| Regulator | Change in –(log p) with CD4 depletion | Regulator | Change in –(log p) with CD8 depletion | |
| IFNG | 40.37 | TNF | 11.03 | |
| LPS | 35.31 | IL27 | 7.98 | |
| Poly I:C RNA | 34.72 | IL10RA | 5.69 | |
| CD40LG | 28.06 | NKX2 | 5.47 | |
| TNF | 24.27 | CpG nucleotide | 5.30 | |
| TLR4 | 23.69 | IFNG | 5.28 | |
| STAT3 | 23.07 | CREBBP | 5.12 | |
| TLR3 | 21.19 | NLRC5 | 5.02 | |
| TICAM | 21.04 | fluticasone | 4.92 | |
| CD40 | 20.32 | IL6 | 4.88 | |
| IL27 | 20.03 | IL27RA | 4.78 | |
| TLR9 | 19.49 | PARP1 | 4.54 | |
| TLR7 | 19.21 | MAPK1 | 3.90 | |
| STAT6 | 17.74 | Mir-21 | 3.86 | |
| TRIM24 | 17.71 | IL1RN | 3.84 | |
| IL10 | 17.38 | SOCS1 | 3.74 | |
| IL1B | 17.12 | STAT5A | 3.67 | |
| TCR | 16.94 | GATA2 | 3.11 | |
| BTK | 16.75 | SMARCA4 | 3.07 | |
| NIKX2–3 | 16.35 | KIT | 2.97 | |
Top 20 Mtb-induced upstream regulators of BAL cell gene expression not associated with Type I interferons. Results are expressed in terms of the decrease in significance (-log p-value) following depletion of CD4+ or CD8+ T cells. As shown, with the exceptions of IFNγ, TNFα, and IL-27 (indicated in bold) the lists do not overlap; further, the reductions in significance of the various regulations is far greater following depletion of CD4+ as compared to CD8+ T-cells.