KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Chicken anti-GFP | Aves Labs | GFP-1020, AB_10000240 |
| Mouse anti bruchpilot (nc82) | Developmental Studies Hybridoma Bank | nc82, AB_2314866 |
| Alexa Fluor 488 Donkey anti-Chicken IgY | Jackson ImmunoResearch Labs | 703–545-155, AB_2340375 |
| Cy3 Donkey anti-mouse IgG (H+L) | Jackson ImmunoResearch Labs | 715–165-150, AB_2340813 |
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| All-trans-retinal | Sigma-Aldrich | R2500 |
| ATP disodium salt hydrate | Sigma-Aldrich | A2383 |
| Critical Commercial Assays | ||
| Luciferase reporter assay | Promega | E1910 |
| Deposited Data | ||
| Drosophila brain single-cell sequencing dataset | [31] | https://doi.org/10.7554/eLife.34550.007 |
| Drosophila brain single-cell sequencing dataset | [32] | GEO: GSE107451 |
| Images of neurons that control mating drive in male Drosophila | This paper | DOI: 10.17632/4c44xb5zyh.1 |
| Experimental Models: Cell Lines | ||
| Experimental Models: Organisms/Strains | ||
| D. melanogaster: w1118/Dp(2;Y), P{hs-hid}Y | Bloomington Drosophila Stock Center (BDSC) | BDSC: 24638 |
| D. m.: wild-type Canton S | [70] | FlyBase: FBsn0000274 |
| D. m.: dsx-Gal4: w1118;;TI{Gal4}dsxKI.Gal4 | [11] | FlyBase: FBti0168641 |
| D. m.: Fru-Gal4: w*;; TI{GAL4}fru[GAL4.P1.D]/TM3, Sb1 | BDSC | BDSC: 66696 |
| D. m.: w*; P{UAS-TrpA1(B).K}attP16 | BDSC | BDSC: 26263 |
| D. m.: CRN-Gal4: w1118; P{GMR42G02-Gal4}attP40 | [26] | N/A |
| D. m.: y1, w*; PinYt/CyO; P{UAS-mCD8::GFP.L}LL6 | BDSC | BDSC: 5130 |
| D. m.: Tsh-Gal80: w1118; P{GAL80}tshGAL80 | Gift from J.H. Simpson | FlyBase: FBti0114123 |
| D. m.: w1118; P{UAS-DenMark}2 | BDSC | BDSC: 33062 |
| D. m.: w1118; P{y[+t7.7] w[+mC]=8xLexAop2-IVS-Syt1::smGdP-HA}su(Hw)attP1 | BDSC | BDSC: 62213 |
| D. m.: w1118; L1/CyO; P{w[+mC]=UAS-DenMark}3, P{w[+mC]=UAS-syt.eGFP}3 | BDSC | BDSC: 33065 |
| D. m.: y1,w*; PBac{y[+mDint2] w[+mC]=13XLexAop2–6XmCherry-HA}VK00018/CyO; Dr1/TM6C, Sb1, Tb1 | BDSC | BDSC: 52272 |
| D. m.: w1118;; P{UAS-IVS-myr::GFP}attP2 | BDSC | BDSC: 32197 |
| D. m.: w1118; P{UAS-eGFP-Kir2.1}/CyO | [71] | FlyBase: FBtp0110871 |
| D. m.: w*;; P{tubP-GAL80ts}2/TM2 | BDSC | BDSC: 7017 |
| D. m.: TH-Gal4: w1118;; P{ple-GAL4.F}3 | [72] | FlyBase: FBti0072936 |
| D. m.: CRN-LexA: w1118; P{GMR42G02-LexA4}attP40 | Gift from Bruce Baker | BDSC: 61540 |
| D. m.: w1118;; P{20xUAS-IVS-GCaMP6s}VK00005 | BDSC | BDSC: 42749 |
| D. m.: w1118; P{LexAop-TrpA1} | [73] | BDSC: FBtp0084378 |
| D. m.: pCd-Gal4: w1118;; P{GMR41A01-GAL4}attP2 | BDSC | 39425 |
| D. m.: pCd-LexA: w1118; P{GMR41A01-LexA}attP40 | BDSC | 54787 |
| D. m.: w1118; P{UAS-ASAP1}attP40 | BDSC | BDSC: 65412 |
| D. m.: w*; PBac{y[+mDint2] w[+mC]=20XUAS-ASAP2s}VK00005 | BDSC | BDSC: 76247 |
| D. m.: w1118; P{y[+t7.7] w[+mC]=13XLexAop2-IVS-CsChrimson.mVenus}attP40 | BDSC | BDSC: 55138 |
| D. m.: w1118;; P{13xLexAop2-IVS-Syn21-CsChrimson-tdTomato}VK00005 | Gift from B.D. Pfeiffer and D.J. Anderson | N/A |
| D. m.: w1118;; P{lexAop-P2X2.Y} | [74] | FlyBase: FBtp0093385 |
| D. m.: w1118, y1;; P{NPF-Gal4.1}1 | BDSC | BDSC: 25682 |
| D. m.: w1118; P{20xUAS>myrTopHat2>Syn21-CsChrimson-tdTomato}su(Hw)attP5 | Gift from B.D. Pfeiffer and D.J. Anderson | N/A |
| D. m.: w*;; TI{FLP}fru[FLP]/TM3, Sb1 | BDSC | BDSC: 66870 |
| D. m.: w1118; P{y[+t7.7] w[+mC]=8XLexAop2-FLPL}attP2 | BDSC | BDSC: 55819 |
| D. m.: w*; wgSp−1/CyO; TI{lexA::VP16}fruP1.LexA/TM6B, Tb1 | BDSC | BDSC: 66698 |
| D. m.: w*;; TI{lexA::p65}dsxlexA::p65 | [75] | Flybase: FBti0201624 |
| D. m.: w*; P{y[+t7.7] w[+mC]=8XLexAop2-IVS-GAL80-WPRE}attP2 | BDSC | BDSC: 32213 |
| D. m.: NPF-RNAi: y1, v1;;P{TRiP.JF02555}attP2 | BDSC | BDSC: 27237 |
| D. m.: w1118; P{UAS-Dcr-2.D}2 | BDSC | BDSC: 24650 |
| D. m.: NPFR-RNAi: w1118; P{KK112704}VIE-260B | VDRC | VDRC: 107663 |
| D. m.: elav-Gal4: P{GawB}elavc155 | BDSC | BDSC: 458 |
| D. m.: w1118; P{13xLexAop2-IVS-Syn21-opGCaMP6s}su(Hw)attP5 | Gift from B.D. Pfeiffer and D.J. Anderson | N/A |
| D. m.: w1118;; P{UAS-Rnor\P2rx2.L} | [76] | FlyBase: FBtp0021869 |
| D. m.: w1118; TH-LexA | [77] | N/A |
| D. m.: +;; DopR2attP | [70] | N/A |
| D. m.: w1118;; P{13xLexAop2-IVS-Syn21-opGCaMP6s}su(Hw)attP1 | Gift from B.D. Pfeiffer and D.J. Anderson | N/A |
| D. m.: w1118;; P{20xUAS-IVS-Syn21-opGCaMP6s}su(Hw)attP1 | Gift from B.D. Pfeiffer and D.J. Anderson | N/A |
| D. m.: w*;; P{y[+t7.7] w[+mC]=10XUAS-IVS-myr::tdTomato}attP2 | BDSC | BDSC: 32221 |
| D. m.: w1118;P{UAS-FLP.U};P{CRE>mCherry>luciferase} | [47] | FlyBase: FBtp0083995, FBtp0065911 |
| D. m.: UAS-CREB2SUP: w1118; P{w[+mC]=dCREB-B-UAS}94 | BDSC | BDSC: 7220 |
| D. m.: UAS-CREB2-A: w*;; P{UAS-CrebB-17A-a.cor}T7.1 | BDSC | BDSC: 9232 |
| D. m.: w1118; P{20xUAS-IVS-Syn21-opGCaMP6s}su(Hw)attP5 | Gift from B.D. Pfeiffer and D.J. Anderson | N/A |
| D. m.: w1118; P{10XUAS-IVS-myr::tdTomato}attP40 | BDSC | BDSC: 32222 |
| D. m.: PP1α96A-RNAi-1: y1, v1; P{y[+t7.7] v[+t1.8]=TRiP.HMS02154}attP40 | BDSC | BDSC: 40906 |
| D. m.: Task7-RNAi: w1118; P{GD3628}v8564 | VDRC | VDRC: 8564 |
| D. m.: w1118;; P{20xUAS-IVS-Syn21-CsChrimson-tdTomato}attP2 | [78] | N/A |
| D. m.: w1118;; P{UAS-IVS-GtACR1}attP2 | [58] | N/A |
| D. m.: w1118; P{UAS-IVS-GCaMP6s}attP40 | BDSC | BDSC: 42746 |
| D. m.: VAChT-RNAi: w1118; P{GD3054}v40918 | VDRC | VDRC: 40918 |
| D. m.: CRN-p65AD: w1118; P{GMR42G02-p65AD}attP40 | This paper | N/A |
| D. m.: w*; P{w[+mC]=UAS-2xEGFP}AH2; P{w[+mC]=ChAT-GAL4.DBD}J8A1 | BDSC | BDSC: 23869 |
| D. m.: w1118; P{UAS-ort.2HA} | [79] | FlyBase: FBtp0093981 |
| D. m.: w1118; P{LexAop-ort.HA} | [80] | FlyBase: FBtp0126450 |
| D. m.: CREB2-RNAi: y1, v1; P{TRiP.HMJ30249}attP40/CyO | BDSC | BDSC: 63681 |
| D. m.: CKIIβ2-RNAi: w1118; P{KK103402}VIE-260B | VDRC | VDRC: 102633 |
| D. m.: PP1α96A-RNAi-2: y1, sc*, v1; P{y[+t7.7] v[+t1.8]=TRiP.HMS02477}attP40 | BDSC | BDSC: 42641 |
| D. m.: P1-B-Gal4: w1118;; P{GMR71G01-GAL4}attP2 | BDSC | BDSC: 39599 |
| D. m.: UAS-CREBDN: w1118;; P{w[+mC]=Cbz}4/TM3, Sb1 Ser1 | BDSC | BDSC: 7222 |
| Oligonucleotides | ||
| FISH probes targeting Task7 (tagged with Quasar-570) | LGC Biosearch Technologies | See Table S3 for sequences |
| FISH probes targeting Dsx (tagged with Quasar-670) | LGC Biosearch Technologies | See Table S3 for sequences |
| Recombinant DNA | ||
| R42G02 DNA fragment | Gift from Gerry Rubin | N/A |
| Software and Algorithms | ||
| ROI-based fluorescence quantification package (MATLAB) | [22] | https://github.com/CrickmoreRoguljaLabs/Blind-image-analysis |
| Courtship-initiation analysis package (MATLAB) | [23] | https://github.com/CrickmoreRoguljaLabs/tapping-codes |
| ROI-based FISH analysis (MATLAB) | This paper | https://github.com/CrickmoreRoguljaLabs/ROI_FISH_analysis |
| Models for fly mating drive dynamics (MATLAB) | This paper | https://github.com/CrickmoreRoguljaLabs |
| Predict putative CREB-regulated genes (MATLAB) | This paper | https://github.com/CrickmoreRoguljaLabs/CREB_analysis |
| FIJI | [81] | https://fiji.sc/ |
| MTrack2 (plugin for FIJI) | Ron Vale lab | https://valelab4.ucsf.edu/~stuurman/IJplugins/MTrack2.html |
| Other | ||
| Phosphate Buffered Saline 10x | MediaTech | 46–013-CM |
| Triton X-100 | Sigma-Aldrich | X100 |
| Potato flakes | Carolina Biological Supply | 173200 |
| Nunc Petri dishes | Thermo Scientific | 150318 |
| Glass-bottom Petri dishes | MatTek | P35G-1.5–20-c |
| 655-nm LED | Luxeon Star LEDs | LXM3-PD01–0350 |
| 530-nm LED | Luxeon Star LEDs | LXML-PM01–0100 |
| Arduino MEGA | Arduino | A000067 |
| Raspberry PI Model B+ | Raspberry PI | Model B+ |
| 2-photon microscope (including the 465-nm LED) | Neurolabware | Standard |