REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Bacterial and Virus Strains
BL21-CodonPlus(DE3)-RIL	Agilent	Cat. #230245
Biological Samples
Rabbit Muscle Acetone Powder	Pel-Freeze Biologicals	Cat. #41995–2
S. pombe Arp2/3 complex	This study	N/A
Chemicals, Peptides, and Recombinant Proteins
Alexa Fluor 568 C5 maleimide	ThermoFisher Scientific	Cat. # A20341
Oregon Green 488 maleimide	ThermoFisher Scientific	Cat. # O6034
(3-Aminopropyl)triethoxysilane (APTES)	MilliporeSigma	Cat. # 440140
EZ-Link-NHS-PEG12-biotin	ThermoFisher Scientific	Cat. # 21312
Methoxy PEG succinimidyl succinate, MW5000	JenKem	Cat. # A3011–1
Biotin-PEG NHS ester, MW5000	JenKem	Cat. # A5027–1
NeutrAvidin	ThermoFisher Scientific	Cat. # 31000
Catalase	Sigma	Cat. # C3515
Glucose Oxidase	MP Biomedicals	Cat. # 195196
Myosin II, rabbit skeletal muscle	Cytoskeleton	Cat. # MYO2
Albumin, from bovine serum	Sigma	Cat. # A2153
cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail tablets	Sigma	Cat. # 11836170001
Zymolyase-100T	Nacalai tesque	Cat. # 07665–55
Salmon Sperm DNA, sheared (10 mg/mL)	Invitrogen	CAS # 7732–18–5
S. pombe Dip1	This study	N/A
S. pombe Dip1(6CysAla)	This study	N/A
S. pombe Wsp1-VCA	This study	N/A
Critical Commercial Assays
In-Fusion HD Cloning Plus CE	Takara	Cat. # 638916
Deposited Data
Experimental Models: Cell Lines
Experimental Models: Organisms/Strains
S. pombe TP150 (protease deficient, h- leu1)	Vladimir Sirotkin	SpBN110
S. pombe VS872 (h- end4::ura4+ ade6-M216, his3-D1, leu1–32, ura4-D18)	Vladimir Sirotkin	SpBN109
S. pombe VS1124a (h+ kanMX6-Pwsp1-mGFP-wsp1, fim1-mCherry-natMX6, ade6-M210, his3-D1, leu1–32, ura4-D18)	Vladimir Sirotkin	SpBN282
S. pombe VS1025–7 (h- kanMX6-Pwsp1-mCherry-wsp1, ade6-M216, his3-D1, leu1–32, ura4-D18)	Vladimir Sirotkin	SpBN259
S. pombe SpBN157–6 (end4::ura4+, kanMX6-Pwsp1-mGFP-wsp1, fim1-mCherry-natMX6, his3-D1, leu1–32, ura4-D18)	This study	SpBN157–6
S. pombe SpBN165–1 (end4::ura4+, dip1-mGFP-kanMX6, fim1-mCherry-natMX6, his3-D1, leu1–32, ura4-D18)	This study	SpBN165–1
S. pombe SpBN278–3 (end4::ura4+, dip1-mNeonGreen-hphMX6, fim1-mCherry-natMX6, his3-D1, leu1–32, ura4-D18)	This study	SpBN278–3
S. pombe SpBN280–1 (h- kanMX6-Pwsp1-mCherry-wsp1, dip1-mNeonGreen-hphMX6, ade6-M216, his3-D1, leu1–32, ura4-D18)	This study	SpBN280–1
Oligonucleotides
Recombinant DNA
pGV67_SpDip1	[7]	Plasmid #174
pGV67_SpDip1(6cysAla)	[8]	Plasmid #180
pGV67_SpWsp1(VCA)-497	[44]	Plasmid #4
pGEX6_NWASP-WA	[45]	Plasmid #222
pRK1043	[46]	N/A
pJK148_SpDip1-mNeonGreen-hphMX6	This study	Pombe vector #181
Software and Algorithms
Fiji/ImageJ	[41]	https://fiji.sc/
MATLAB	MathWorks	N/A
ImageJ filament tracking plugin	Gift from Jeff Kuhn	N/A
ImageJ StackProfileData macro	Michael Schmid	https://imagej.nih.gov/ij/macros/StackProfileData.txt
Other
Amicon Ultra-4 Centrifugal Filters 10,000 MWCO	MilliporeSigma	Cat. # UFC9010
Vivaspin Turbo 15 30,000 MWCO	Sartorius	Cat. # VS15T21
Vivaspin Turbo 15 3,500 MWCO	Sartorius	Cat. # VS15T91
Richard-Allan Scientific Slip-Rite Cover Glass (24 × 60 #1.5 coverslips)	ThermoFisher Scientific	Cat. # 152460