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. Author manuscript; available in PMC: 2020 Oct 7.
Published in final edited form as: Dev Cell. 2019 Aug 29;51(1):89–98.e4. doi: 10.1016/j.devcel.2019.08.002

Figure 2. Sema3a is expressed by type I and IIa myofibers but not type IIb myofibers.

Figure 2.

(A) (Top) Transverse sections of soleus muscle from 3-month-old C57Bl6 mice were co-immunostained for Sema3a (red), type IIa myosin (green), and type I myosin (magenta). (Bottom) Transverse sections of gastrocnemius muscles were co-immunostained for Sema3a (red), Type IIb myosin (green), wheat-germ agglutinin (white), and Hoechst (blue). Scale bar: 50 µm. (B) Transverse gastrocnemius muscle sections of Tw2-CreERT2; R26-tdTO mice at 4 months post-TMX were fixed and immunostained for Sema3a (green). Sema3a staining was over-layed with tdTO signal from myofibers receiving Tw2+ cell contribution (red) and counterstained with Hoechst (blue). Scale bar: 50 µm. (C) Transverse sections of tongue muscle from 3-month-old C57Bl6 mice were co-immunostained for Sema3a (red), type IIb myosin (green), wheat-germ agglutinin (magenta), and Hoechst (blue). Scale: 50 µm. See also Figure S2