Skip to main content
. Author manuscript; available in PMC: 2020 Oct 7.
Published in final edited form as: Dev Cell. 2019 Aug 29;51(1):89–98.e4. doi: 10.1016/j.devcel.2019.08.002

Figure 3. Tw2-MB and Pax7-MB are differentially responsive to Sema3a stripes.

Figure 3.

(A) Schematic of Sema3a stripe assay. Sema3 stripes are shown in green and cells are shown in red. (B) (Top) Tw2-MB (red) were seeded onto Sema3a stripes (green) and analyzed one day after seeding. (Bottom) Pax7-MB (red) were seeded onto on Sema3a stripes (green) and analyzed one day after seeding. Cells were co-stained with Hoechst (blue). Scale bar: 100 µm. (C) Quantification of Sema3a avoidance as the percent of cells residing off the stripe over total cells in the field. The dashed line represents the baseline for cells unresponsive to Sema3a stripes. Three separate fields were quantified for each sample with a total of 3 samples per cell type. *: p < 0.05. (D) Tw2-MB (red) were differentiated 1-day after seeding onto Sema3a stripes (green). Cells were fixed after 4 days in DM and co-stained with Hoechst (blue). See also Figure S3. Source data for 3C is provided in Supplementary Table 1.