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. Author manuscript; available in PMC: 2020 Oct 7.
Published in final edited form as: Dev Cell. 2019 Aug 29;51(1):89–98.e4. doi: 10.1016/j.devcel.2019.08.002

Figure 7. Mck-Sema3a Transgenic mice prevent Tw2+ cells from contributing to type IIb fibers.

Figure 7.

(A) Schematic of Tw2+ cell lineage tracing in mck-Sema3a transgenic mice. Tw2-CreERT2; tdTO and mck-Sema3a Tg; Tw2-CreERT2; R26-tdTO mice were injected with 3 doses of TMX on 3 alternating days starting at 8 weeks of age. Muscles from these mice were then harvested 8 weeks later. (B) Transverse gastrocnemius muscle sections of wild-type C57Bl6 and mck-Sema3a transgenic littermates were immunostained for Sem3a (red), type IIb myofibers (green), wheat-germ agglutinin (white), and Hoechst (blue). Scale: 50 µm C) Immunostaining of gastrocnemius muscle from Tw2-CreERT2; tdTO mice and mck-Sema3a; Tw2-CreERT2; tdTO mice for type IIb fibers (green) and tdTO (red). Scale: 50µm. (D) Quantification of the percent of tdTO+ type IIb fibers over total type IIb fibers. 500 type IIb myofibers were quantified per mouse (Tw2-CreERT2; tdTO, n = 3 mice; mck-Sema3a; Tw2-CreERT2; tdTO, n = 4 mice). ****: p < 0.00005. See also Figure S7. Source data for 7D is provided in Supplementary Table 1.