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. 2019 Oct 3;12:8125–8138. doi: 10.2147/OTT.S224721

Figure 2.

Figure 2

High levels of CXCL8 promote glioma cells proliferation. (A, B) The over-expression efficiency against CXCL8 was verified by qRT-PCR and Western blotting in U-251MG and U-87MG cells. (C) Growth curves between Vector and CXCL8 (OE) by CCK-8 assay. The results are shown as the Mean ± Standard Deviation (SD) of three independent experiments. (D, E) Representative images (left panels) and histogram quantification (right panels) of the EdU (D) and colony formation assay (E) with U-251MG and U-87MG cells. Statistical significance was assessed using two-tailed Student’s t test (A, C) and one-way ANOVA followed by Dunnett’s tests for multiple comparisons (D, E). Scale bars: 50 μm. **p < 0.01 and ***p < 0.001 or ##p < 0.01 and ###p < 0.001.